General Cell Based Assays
Sigma Aldrich Fine Chemicals Biosciences Aspartate Assay Kit suffic
Aspartate the carboxylate anion of aspartic acid is an acidic non-essential amino acid involved in protein synthesis and multiple other cellular biochemical pathways. Aspartate contributes to nucleotide synthesis via the synthesis of the precursor inosine mono-phosphate. In the urea cycle aspartate is a key metabolite donating a nitrogen group towards the formation of urea. Aspartate is also critical for oxidative phosphorylation as part of the aspartate-malate shuttle which transfers reducing equivalents across the mitochondrial membrane.
ABclonal Technology GCGR Rabbit pAb
The protein encoded by this gene is a glucagon receptor that is important in controlling blood glucose levels. Defects in this gene are a cause of non-insulin-dependent diabetes mellitus (NIDDM).
Montana Molecular Borealis Arrestin Assay - B2AR D2040G, CMV, 10mL
Use the B2AR Borealis arrestin assay to measure arrestin signaling kinetics with an unmodified B2AR receptor. Add the receptor and fluorescent sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Compare with cAMP signaling responses using Montana Molecular’s cADDis cAMP assay. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells. Each kit includes the sensor, receptor, GRK, and positive control agonist.
Molecular Devices Assay Kit, Calcium 6-QF, Quench-Free, Bulk, 100 Plates
The FLIPR Calcium 6-QF bulk assay kit is a quench-free, fluorescence-based reagent set for measuring intracellular calcium flux in high-throughput screening. The QF formulation is optimized for quench-sensitive targets and enables multiplexing with luminescent assays while minimizing probenecid use.
- Quench-free formulation suitable for quench-sensitive assays and multiplexing.
- Bulk kit sufficient for 100 96-well or 384-well plates, or 150 1536-well plates.
- Includes 10 vials of Component A and 10 vials of Component C, each vial covering multiple plates.
- Assay buffer not included; HBSS plus 20 mM HEPES is recommended as a substitute.
- Compatible with high-throughput FLIPR and FlexStation microplate readers.
- Store reagents at -20°C and other components at 4°C; reagents stable for six months in original packaging.
Aat Bioquest MitoROS™ 580 *Optimized for Detecting Reactive Oxygen Species (ROS) in Mitochondria*
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons.
Aat Bioquest Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*
The detection of intracellular hydroxyl radical is of central importance to understanding proper cellular redox regulation and the impact of its dysregulation on various pathologies.
Cayman Chemical DUB ActIvIty asy KIt 96Wel
Cayman’s DUB Activity Assay Kit facilitates the rapid, robust measurement of deubiquitinating enzyme activity in vitro. The kit utilizes a high purity, fluorogenic substrate (ubiquitin-AMC) together with suitable calibration standards and controls for the accurate and sensitive assessment of DUB activity. Continuous kinetic or end-point assays can be performed in a 96-well plate format for multi-sample analysis. EAch kit contains sufficient material for one full 96-well plate assay set-up to be run. Ubiquitin-AMC is not a suitable substrate for all DUBs. Compatibility must be determined by the end user.
Eurofins Discoverx InCELL Hunter eXpress CSF1R Kinase Binding Assay, 2pl Kit. (96-0047E15CP0M)
The InCELL Hunter eXpress CSF1R Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of CSF1R via detection of CSF1R levels. This product uses EFC technology. The cell line included overexpresses CSF1R target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.